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Last updated May 2006.
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EQUIPMENT
How to build an inebriometer PDF (908 KB)
ASSAYS
Inebriometer use PDF (60 KB)
Inebriometer cleaning PDF (60 KB)
Booze-o-mat use: Tracking protocol PDF (84 KB)
Booze-o-mat use: Video settings PDF (88 KB)
Ethanol absorption assay PDF (544 KB)
Protein assay PDF (68 KB)
Lifespan experiment PDF (68 KB)
Negative geotaxis "Bang" assay PDF (96 KB)
Dopamine & 3IY feeding and locomotor assays PDF (76 KB)
HISTOLOGY
Adult CNS dissection protocol PDF (1.7 MB)
Larval CNS dissection protocol PDF (188 KB)
Antibody staining protocol PDF (48 KB)
Dissection & antibody staining of
3rd instar larval bodywall or NMJ PDF (568 KB)
Beta-galactosidase activity staining in CNS PDF (52 KB)
Frozen head sections PDF (252 KB)
MUTAGENESIS
Ethyl methanesulfonate (EMS) mutagenesis PDF (116 KB)
Hydroxyurea ablation of mushroom bodies
PDF (84 KB)
PREPS
Fly food recipe PDF (76 KB)